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The nanobody (Nb) 2Rs15d 53 with an N-terminal Gly-Pap-GG tag was expressed in the cytoplasm of NEBExpress(lowRF1) and purified as described above for AmpC

Samples were then either snap-frozen in liquid nitrogen and stored at 20 C before analysis, or immediately injected to the mass spectrometer (Extended Data Fig

Beyond macrophages and neutrophils, other immune cells such as dendritic cells and T and B lymphocytes may also participate in ferroptosis-related immune regulation in periodontitis, collectively forming a multilayered and interconnected immune-ferroptosis network

The major treatments for iron overload include phlebotomy and chelation therapy Means for both the early detection and treatment of iron overload are fortunately available now and, with appropriate therapy, many of the pathological effects of iron excess can be avoided