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The samples were permeabilized with PBS 0.4% Triton X-100 for 4 min, washed with PBS and blocked for 1 h at room temperature with 5% BSA in PBS

Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses for differentially expressed CsGSTs under cold stress revealed that these genes responded to cold stress probably through glutathione metabolism. Finally, we screened seven candidates that may be involved in cold stress using Venn analysis, and their promoters were analyzed using PlantCARE and New PLACE tools to predict the factors regulating these genes

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Taken together, the above-mentioned data suggest that GSH might serve as an important wound signal that triggers wound-induced systemic [Ca 2+ ] cyt transmission, activates JA biosynthesis, and regulates plant defense responses