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doi:10.1186/s40168-023-01569-z

Glutathione transferase enzyme assays Frozen tissue samples were pulverized in liquid nitrogen and extracted in 5:1 (v/w) of 50 mM Tris-HCl (pH 7.5) containing 2 mM EDTA, 1 mM dithiothreitol (DTT) and 2% (w/v) polyvinylpolypyrrolidone

Without a placebo arm or comparison group receiving the procedure without copper peptides, it is impossible to isolate how much of the improvement comes from each individual ingredient versus the combination and delivery method