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Thereafter, horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Jackson Immunoresearch, West Grove, PA) was diluted in PBS+1%BSA to a final dilution of 1:1,000, and 50 L of this dilution was added to each well in the microtiter plates

MTHFR Gene Variants with Hyperhomocysteinemia and Cardiovascular Diseases Elevated Hcy levels are considered an independent risk factor for the development of CVD, which encompasses CAD, MI, IS, hypertension, and thrombosis [84,85]

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Low protein intake is associated with a major reduction in IGF-1, cancer, and overall mortality in the 65 and younger but not older population