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Future research should focus on identifying pain-specific biomarkers, refining diagnostic criteria, and integrating multi-omics data and neuroimaging techniques to better distinguish pain mechanisms in IBS-C versus FC and develop more precise, patient-centered interventions

RNA isolation and quantitative real-time PCR Total RNAs were isolated from liquid nitrogen-disrupted seedling leaves using Trizol reagent (Invitrogen, Carlsbad, CA, USA), and by treatment with RNase-free DNase I (Promega, Madison, WI, USA)

The animal research is more consistent than the human research

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