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Anesthesia: Used before surgery to reduce saliva and respiratory or gastric secretions, and during procedures to block cardiac vagal reflexes and manage drug-induced arrhythmias

Neural stem cell differentiation into astrocytes and, at a lower extent, into new neurons is regulated by some factors such as bone marrow proteins (BMPs), whose expression is upregulated in the injury lesion after SCI (Wang et al., 2011

Measurement of oxidatively induced DNA damage and its repair, by mass spectrometric techniques

Statistical analysis Sample sizes for single-nucleus transcriptomics and DREADD experiments were determined based on prior experience with similar paradigms 12,13