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Cell suspensions were then applied to the column, and the flow-through was collected in a new 15-ml tube

Mechanistically, SRT1720 reduces oxidative stress by decreasing the levels of MDA, TGF-1/connective tissue growth factor (CTGF), and glutathione (GSH), increasing the levels of SOD, GPX, thereby protecting cells from ROS-induced damage and playing a protective role against UUO-induced tubulointerstitial fibrosis (Ren et al., 2017)

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