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Appl Microbiol Biotechnol 87:137144 Zhao J, Zhu W-H, Hu Q (2001) Selection of fungal elicitors to increase indole alkaloid accumulation in Catharanthus roseus suspension cell culture

It required 30 ml of elution buffer to elute all the protein and biomolecules from the column and the researcher made a mistake and collected only three 10 ml fractions