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10.1126/science.1098837 Science 32 HidegE.KalaiT.KosP

After tumor spheroids were visible in culture, brightfield microscopy images ( 20 magnification) were obtained using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, VT)

Protein-membrane binding assay For this assay, sodium carbonate-treated PM fractions (2 mg/ml) from WT and hyh mice were incubated with recombinant -SNAP (WT or M105I) to a final concentration of 3 M in 200 l NB buffer