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We calculated the mean fluorescent intensity (MFI) using ImageJ software as described in Methods

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Nat Genet 39(10):12561260

Sometimes, skin or blood tests may be used to identify underlying causes

Keller A, Rimestad ML, Friis Rohde J, Holm Petersen B, Bruun Korfitsen C, Tarp S et al (2021) The effect of a combined gluten- and casein-free diet on children and adolescents with autism spectrum disorders: a systematic review and meta-analysis