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Nature 423 , 720724 (2003)

A detailed protocol for northern-blot analysis of small RNAs is available at Results were analysed on a Typhoon phosphorimager, using ImageQuant TL (v.10.2) for quantification of band intensities

Strengthening the endogenous C5 pathway by sequentially knocking out the negative regulatory protein gene hemX , overexpressing the endogenous glutamyl-tRNA reductase gene hemA , and knocking out the glutamate dehydrogenase gene rocG , increased the heme production by 427%

Abstract Background Direct cardiac reprogramming offers a promising strategy to regenerate cardiomyocytes for heart repair, but its efficiency and maturation remain limited