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For growth assays performed in 96-well plates, 200 L of culture was aliquoted in duplicate wells

This study, being a meta-analysis and including randomized controlled trials, makes the findings stronger compared to other studies

Rhim HC, Kim MS, Park YJ, Choi WS, Park HK, Kim HG, Kim A, Paick SH

Phosphorylated CREB translocates to the nucleus where it binds to cAMP response elements (CREs) in DNA, upregulating transcription of the growth hormone gene and other genes involved in GH synthesis and secretion