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LockeLWCrouserEDWhitePJulianMWCaceresEGPappACet al

Aust N Z J Psychiatry 2017;51:241-9

Characterization of methionine dependence in melanoma cells

The brain was removed and maintained with 4% paraformaldehyde for 48 h before dehydrated with 75, 80, 95 and 100% alcohol and thereafter transparent with mice xylene, dipped in wax, and sliced into brain sections (5 m thickness) with a microtome (Leica, Germany)