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Where required, cells were treated with 1 M setanaxib, 10 M sulforaphane or 10 M tert-butylhydroquinone as specified in Figure legends

The most common side effects include nausea (44% of patients), diarrhea (30%), vomiting (24%), and constipation (24%), according to the Wegovy prescribing information

Methylation Capacity Second, NNMT inhibition affects cellular methylation capacity: SAM is required for hundreds of methyltransferase reactions throughout the cell, and NNMT's consumption of SAM can deplete this critical resource, while the SAH produced by NNMT is a potent product inhibitor of many methyltransferases