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After HS, the HSR takes effect, resulting in increased expression of HSP

Two-fold concentrated stock solutions containing either 2 mM Aldrithriol-2 (Sigma-Aldrich, Darmstadt, Germany, CAS-2127-03-9, AT-2) (oxidation control), 10 mM Dithiothreitol (Sigma-Aldrich, Darmstadt, Germany, CAS-3483-12-3, DTT) (reduction control), 5 mM reduced glutathione (GSH, Sigma-Aldrich, CAS-70-18-8, pH 7.4 in 1 M HEPES), 5 mM oxidized Glutathione (GSSG, Sigma-Aldrich, CAS-27025-41-8, pH 7.4 in 1 M HEPES) or HEPES were prepared freshly

PE-A5% is performed using Albutein 5% (Grifols)

The nanoparticles were fabricated using the nanoprecipitation technique, and the resulting RH-PLGA/chitosan nanoparticles had an average size of 468.0 nm and a PDI of 0.29