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doi: 10.1038/nrneph.2016.41 26 OchandoJOrdikhaniFBorosPJordanS

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Flux along this pathway is likely to be increased under general conditions in which the mitochondrial NADH:NAD + ratio increases, which would include hypoxia and where there are deficiencies in NADH oxidation due to mitochondrial dysfunction

This molecule was duplexed and amplified by 5 cycles of PCR using Phusion HF polymerase (New England Biolabs, NEB), then inserted into blunt II TOPO vector (Invitrogen) to generate a plasmid for electroporation efficiency control (pSSBR)