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Trials conducted between 2001 and 2007 demonstrated statistically significant reductions in body fat, although the larger Phase IIb OPTIONS trial did not replicate these findings at sufficient magnitude for regulatory approval, and formal development was discontinued in 2007

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After preheating at 37 C for 5 min of the reaction mixture (500 L) with 0.1 M NaCl (350 L) and plant extract (50 L), the enzyme reaction was initiated by adding 100 L of 100 U/ml ADH (Merck, Germany) for NAD(H) determination, or 100 L of 14 U/ml G6PDH (Merck, Germany) for NADP(H) determination

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