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demonstrated that overexpression of phosphoglycerate dehydrogenase (PHGDH) attenuated ferroptosis by modulating the p53/SLC7A11 signaling axis, thereby reducing calcification in human coronary artery smooth muscle cells (HCASMCs)

Proteins were then precipitated by addition of 500 l methanol (pre-cooled at 80 C), 400 l LCMS-grade water, and 100 l chloroform, and the mixture was vortexed and centrifuged (10,000 g, 10 min, 4C) to afford a protein disc at the interface of CHCl3 and aqueous layers

Nongenerative artificial intelligence in medicine: advancements and applications in supervised and unsupervised machine learning