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TMAO and chronic kidney disease (CKD) Gut dysbiosis is linked with an increased risk of kidney disease, particularly when TMAO and choline levels are elevated (Velasquez et al

Suspensions were centrifuged at 5,000 g for 5 min at 4C, supernatants were transferred to a new tube, and TCA buffer was added to a final concentration of 33% and incubated at 4C overnight to precipitate histone proteins