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The grayscale values of the target protein bands were analyzed with ImageJ software, and relative expression levels were calculated by using GAPDH as an internal control

Most studies focus on its antioxidant role in cellular health, which indirectly supports follicle function

In B12 deficiency, DNA synthesis in red blood cell precursors (erythroblasts) can be impaired, preventing the normal maturation and division of these cells, resulting in larger-than-normal red blood cells

This dual dysfunction reduces glucose uptake and antioxidant defense while paradoxically activating mTORC1 to promote cellular senescence (32, 33)