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The beads were washed four times with high-salt wash buffer (10 mM HEPES, pH 7.9, 25% glycerol, 1.5 mM MgCl 2 , 300 mM KCl, and 0.1% Triton X-100), followed by two washes with low-salt wash buffer (10 mM HEPES, pH 7.9, 25% glycerol, 1.5 mM MgCl 2 , 100 mM KCl, and 0.1% Triton X-100)

The body tightly regulates intracellular glutathione levels along with its synthesis from amino acid precursors

Zhang et al., 2024) identify GPX4 as a key enzyme counteracting LPO in hippocampal neurons, closely linked to AD neurodegeneration

Moreover, caffeine has been shown to inhibit chronic inflammation, its anti-inflammatory nature downregulates the pro-inflammatory cytokines and microglial activation, thereby protecting neuronal damage (156)