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For caspase-4-GSDMD-induced liposome leakage, 0.2 M recombinant human His-sumo-GSDMD was pretreated with indicated concentrations of MeCbl for 0.5 h, then incubated with 0.2 M active-caspase-4 for another 2 h, 200 M liposome was then added into the caspase-4-GSDMD reaction system and incubated at 37 C overnight for final fluorescence measurements

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Both natural FET cycles resulted in pregnancies, and all pregnancies were achieved by blastocyst transfer, consistent with current clinical knowledge regarding the advantages of blastocyst-stage embryos in improving implantation outcomes

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