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(Liu is on scientific advisory board of the University of Oxfords Jenner Institute, which developed AstraZenecas vaccine for COVID-19.) Had this to say in The Scientist

Following sonication, all sample tubes were incubated on ice for 10 min, then centrifuged for 10 min at 15,000 xg at 4 C

In addition to resistance to diet-induced obesity, the absence of HSL also protects against genetic obesity, based on the observation that mice homozygous for both HSL and leptin deficiency ( ob/ob ) are 26% lighter than ob/ob mice and this is reflected by a reduction in WAT mass [14]