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Reconstitution and Handling GHK-Cu is supplied as a lyophilised powder (usually as the copper complex) in research-grade vials

Cells in 6-well plates were labeled with 2 M BODIPY for 30 min, trypsinized, strained, resuspended in FACS buffer, and analyzed by flow cytometry (FITC/Texas Red channels on a BD LSRFortessa TM cell analyzer)

These timepoints were selected to represent distinct stages of iHLO development and maturation: Day 7 signifies the hepatoblast differentiation stage, Day 15 marks the early hepatocyte stage, and Day 30 and Day 45 indicate progressively matured liver organoid stages with increasing liver cell populations and functions

Their reactivity is based on the 1,3-dicarbonyl chemistry originally demonstrated with dimedone