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A nongray theory of extrasolar giant planets and brown dwarfs

Horowitz S, Horowitz A, Nilsen TW, Munns TW, Rottman FM

For IP reactions with endogenous G6PD, the supernatants were pre-cleared with protein A/G-coupled agarose (Thermo Fisher Scientific), anti-G6PD antibody was added to the cell lysates and stirred at 4 C overnight, and then protein A/G-coupled agarose were added and incubated for 2 h, followed by washing 4 times and boiling in 5X loading buffer