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Interestingly, despite the accumulated levels of lipids, at 20 C, only RNAi knockdown of glp-1 and iff-1 led to an increase in lifespan, whereas knockdown of mog-3, fbf-1/2, mpk-1, gld-1 , and pro-1 resulted in shortened lifespan, and inactivation of fem-1, fem-3 and fog-3 showed no change in lifespan compared to wild-type N2 (WT) (Supplementary Fig