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We selected AsPC-1 as the cell line for our studies, because this is an aggressive metastatic cell line that allows simulation of the most common scenario in the clinic, that is, a tumour that has already metastasised at the time of diagnosis

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This B12-IF complex reaches the terminal ileum, where it binds to heterodimeric receptors cubam composed of an outer protein cubulin and transmembrane protein amnionless , located on the surface of polarized epithelial enterocytes in the apical brush border (Fyfe et al., 2004)