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While MSI testing through PCR methods is an alternative, it is considered more laborious and less informative regarding which specific MMR genes are affected ( The guidelines suggest that the combination of IHC testing followed by MLH1 promoter testing is likely to be the most cost-effective approach for identifying Lynch syndrome in patients with endometrial cancer ( In summary, IHC is consistently recommended across all guidelines as the first diagnostic step for LS

Rev Soc Bras Med Trop (2021) 54:e01862021

RNA from liver tissue was extracted using Trizol reagent (Invitrogen, Cergy Pontoise, France)

MDM2 and MDMX are negative regulatory proteins of the P53 gene