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Lifeng et al

Since the inception of using glutathione as a capture agent for GST fusion proteins, almost all scientific supply companies carry a variation of glutathione conjugated polymeric beads, and at least one patent has been awarded [8] for its utility in proteins

Luciferase activity was measured at 48 h after transfection utilizing the Dual-Luciferase Reporter Assay System (Promega Corporation, Madison, WI, USA)

In particular, Akt phosphorylation, compared to ERK phosphorylation, appeared to be more sensitive to the phosphorylation of Met, which is evident by the dramatic reduction in pAkt in the case without 51 (Fig