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Nat Rev Rheumatol (2020) 16(9):51424

A brief review of radiolabelling nucleic acid-based molecules for tracking and monitoring

The wild-type or mutant vanR genes were amplified using plasmid pVanR as template with primers VanR -for- EcoR I and VanR -rev- Xho I, and ligated into plasmid pGEX-6p-1 after digestion with EcoR I and Xho I, resulting in plasmid pGEX-6p-1- vanR carrying wild-type or mutant vanR gene