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The study used 200 mg ALA through the two administration routes to determine the pharmacokinetic parameters of ALA

Sajeesh S, Broekelman T, Mecham RP, Ramamurthi A

Preclinical research has indicated that Withania somnifera elicits antidepressant-like effects that are comparable to those of imipramine and fluoxetine, thereby corroborating its involvement in serotonergic and noradrenergic signaling cascades (78)

The composition of the sample/lysis buffer also needs to be compatible with the subsequent purification step(s) if the investigator is to avoid time-consuming buffer exchange steps before column chromatography