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Given the inhibitory activities of R001 on both G6PD and TrxR1 functions, we investigated the effects of the natural product on cellular NADPH and GSH levels using commercially available assay kits

[6] GSH directly protects DNA from oxidative damage and indirectly supports nucleotide excision repair via redox regulation of involved enzymes

According to these authors, during the parasite transformation from the insect to infective trypomastigote form, mitochondrial organelle undergoes remodeling that results in (a) increased activities of the respiratory complex II and complex III facilitating electrons' entrance to the ETC, and (b) decreased expression and activity of complex IV

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