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When the tumor size extended over 100 mm, treatments were conducted by diluting 0.5 mg of each drug in 500 l PBS

Only cells that remained within the field of view throughout the entire recording period, were sufficiently separated to allow accurate segmentation, and did not overlap with neighboring cells were included

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They express surface antigens that induce T-cell proliferation, carry the catalytically active TNF- converting enzyme, and promote the inflammatory response (46, 50)