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More relevant for targeting ECD was the study of bloodendothelium interactions, where HUVECs were divided into untreated (quiescent endothelium) and stimulated with phorbol-12-myristate-13-acetate (PMA) to mimic an inflammatory response before whole blood perfusion

Gerli et al., 1990), see Figure 1

PCR amplicons were separated by electrophoresis on 1% agarose gel (40 min, 100 V), stained with gelred (Biotium, So Paulo, SP, Brazil), and visualized under ultraviolet light