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Additionally, we identified 8 Wnt ligand antagonists with peak expressions during the anhepatic stage, along with the Wnt signaling co-receptor LRP5, which showed the lowest expression during the anhepatic stage

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PCR amplicons were separated by electrophoresis on 1% agarose gel (40 min, 100 V), stained with gelred (Biotium, So Paulo, SP, Brazil), and visualized under ultraviolet light