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Exploring the Potential of Bioactive Peptides: From Natural Sources to Therapeutics PMC [2] Abuchowski A, van Es T, Palczuk NC, Davis FF

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The supernatants of the cell lysates were isolated at 12,500 g for 30 min at 4 C and were incubated with anti-Flag magnetic beads overnight at 4 C, followed by extensive washing with NP-40 Lysis Buffer