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10.1016/j.ecoenv.2019.109425 64 DingZ.WuX.WangY.JiS.ZhangW.KangJ.et al (2020)

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For endogenous immunoprecipitation, the cells were lysed, the lysates were incubated with anti-WDR24 antibody (rabbit IgG used as control) and protein A/G magnetic beads (MCE) overnight at 4 C, washed five times with 0.1% Tween-20 and boiled with SDS loading buffer for 10 min 68