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Pull-down assay Five micrograms of the bait protein c-MET927-TSI3-His was immobilized to TSE resin by incubating for 30 min on ice and washed with binding buffer PBS (Gibco) supplemented with10 M Tinzaparin and 2 mM CaCl2

The identification of these pathways has also led to the classification of asthma into distinct inflammatory endotypes and has facilitated the development of targeted biologic therapies directed against IL-5, IL-4/IL-13 signaling, and IgE, highlighting the central role of eosinophils and T2 immune responses in asthma pathophysiology (Sahnoon et al

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