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Description

Lefkovits initially followed the Dutton-Mishell protocol which used 1ml macrocultures, but subsequently 'began to dilute macrocultures to obtain microcultures' (figure 22)

PCOS is one of the most common causes of anovulation in women (50)

The secondary antibodies (listed in Additional file 3: Table S3) employed to visualize the localization of primary antibodies were Cy3-conjugated goat anti-rabbit IgG (1:1000)