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The reaction was initiated by adding 4 L of 25 M stock purified NNMT protein (final concentration of NNMT in the reaction was 200 nM) and incubated on a heat block at 37 C

Most supplements contain either vitamin D2 or vitamin D3

Customized supplementation plans targeting genetic deficiencies in vitamins, minerals, and antioxidants like methylated B vitamins for improved detoxification and optimising methylation