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Whole eyes were extracted, fixed in 4% PFA for 30 min, and then cryoprotected in 30% sucrose with 0.02% sodium azide overnight

As a practical example, network pharmacology can first overlap PD oxidative-stress gene modules with target sets of MFH actives to nominate shortlists of 23 component pairs (e.g., Rg1 + EGCG, or APS + CUR) that co-cover Nrf2/ARE and PI3K/Akt while dampening NF-B

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