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Absorbance was measured with a microplate reader (Epoch, BioTek Instruments, VT, USA)

Archives of Neurology, 52 (7), 717724

doi: 10.1111/j.1572-0241.2003.07385.x

Solution : To enhance protein stability, it is essential to use gentle purification conditions and include stabilizing agents in buffers, such as protease inhibitors or stabilizers