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Then, the mixture was aliquoted into a Petri culture dish and incubated at 37 C for 20 min allowing for hydrogel formation, followed by culturing for 48 h in the complete culture medium (-MEM supplemented with 10% FBS, 1% antibiotics, 2 mM L-glutamine, 100 mM NEAA, and 550 M 2-ME) in the absence of extra mitogens, e.g., basic fibroblast growth factor (bFGF) and platelet-derived growth factor AA (PDGF-AA), neurotrophic factors, e.g., heregulin--1, and chemicals, e.g., all trans-retinoic acid (RA) and forskolin, all of which are commonly used for the induction of Schwann-like cells from MSCs [33, 34]

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Both contain the same active ingredient, tirzepedite

The new NICE guidelines recommend healthcare professionals offer B12 blood tests to anyone with at least one symptom or sign and one or more risk factors