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Cells were collected and lysed in Pierce IP Lysis Buffer (#87788, Thermo Scientific, Waltham, MA, USA) for 30 min at 4 C, followed by centrifugation at 12,000 g for 20 min

TLR9endosomal sensing of CpG DNA TLR9 is a specialized PRR that evolved to detect unmethylated CpG motifs, which are abundant in bacterial DNA and mtDNA but relatively rare and typically methylated in nuclear DNA [123, 124]

Coinfection with recombinant vaccinia viruses expressing poliovirus P1 and P3 proteins results in polyprotein processing and formation of empty capsid structures