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To further investigate whether lysosomal LPO is responsible for ferroptosis induction, we synthesized a lysosome-targeting probe based on NBD-Pen with morpholine (a lysosomal targeting group) 16 , named 2,2,6-trimethyl-4-(2-morpholinoethyl)(7-nitrobenzo[ c ][1,2,5]oxadiazol-4-yl)amino-6-pentylpiperidine-1-oxyl (Lyso-NBD-Pen) (more polar diastereomer 1, less polar diastereomer 2) (Supplementary Fig

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However, the immunosuppressive tumor microenvironment, particularly the presence of MDSCs and regulatory T cells, limits the effectiveness of ICB therapy