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Description

- tubulin, A2228 from Sigma-Aldrich, Saint-Quentin-Fallavier, France, dilution 1/10,000), Supersignal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific, Illkirch-Graffenstaden, France), RNeasy Mini kit (Qiagen, Valencia, CA, USA), iScript cDNA Synthesis Kit (Bio-Rad), FG Power SYBR Green (Thermo Fischer Scientific, Illkirch-Graffenstaden, France), iCycler iQ Real-Time Detection System (Bio-Rad, Marnes-la-Coquette, France), MESA Green qPCR Mastermix (Eurogentec, Uppsala, Sweden), 2,7-dichlorodihydrofluoresceindiacetate (H 2 DCFDA) from Sigma-Aldrich GmbH (CAS No.: 4091-99-0, Taufkirchen, Germany)

The GHRP triggers the pulse, the GHRH analog amplifies it

November 12, 2020

Identifying the analogues of berberine as promising antitubercular drugs targeting Mtb-FtsZ polymerisation through ligand-based virtual screening and molecular dynamics simulations