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Samples were blocked with 5% bovine serum albumin (BSA) at room temperature for 1 h and subsequently incubated with primary antibodies against vimentin (1:100, Abcam, ab8978, Cambridge, UK), anti-cytokeratin 7 (1:100, Abcam, ab68459, Cambridge, UK), anti-VEGFA (1:100), and anti-IL8 (1:100) overnight at 4 C