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In many such patients, diazoxide is ineffective, resulting in the need for a trial of SRL to reduce excess insulin secretion, before resorting to irreversible sub-total pancreatectomy with consequent development of insulin dependent diabetes and exocrine pancreatic insufficiency

The tissue was then incubated in Hanks buffered salt solution (HBSS) containing 15 mM HEPES, 50 U/mL collagenase type II (Thermo Fisher 17101015), and 100 U/mL hyaluronidase (Sigma Aldrich H3506) at 37 C for 30 min

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The other predictors of ECOPD reported are poor exercise tolerance as measured by the 6-min walk distance (6MWD) test or the five-times sit-to-stand test (5STS) 31 , reduced hand-grip strength 32 , frailty measured by the 4-meter gait speed test 33 and increased IgE levels 34,35