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Matrices listed below were spiked with certain level of recombinant GPX4 and the recovery rates were calculated by comparing the measured value to the expected amount of GPX4 in samples.

10.3389/fphys.2020.00054 141

Biochemical and histological analyses were performed on the hippocampal and cortical tissue, assessing oxidative stress markers, antioxidant enzyme activity, and glial activation through spectrophotometric assays and immunohistochemistry

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