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A total of 100 nM ABHD11-Flag was added to 100 M of the substrates (OGDHc-E2 peptides or succinyl-CoA) in assay buffer (50 mM TrisHCl pH 7.4, 100 mM NaCl) in 1.5 ml Eppendorf tubes for each condition on ice

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They found that the ferroptosis of murine fibrosarcoma MCA205 cells could increase the number of BMDCs with mature phenotype and exert a vaccine-like action in vivo, which suggested that ferroptosis could trigger an innate and adaptive immune response

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